Associate Scientist Job at Pacer Group, South San Francisco, CA

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  • Pacer Group
  • South San Francisco, CA

Job Description

Job Title: Scientist - II

Duration: 12 Months

Location: South San Francisco, CA 94080

Summary:

Education and experience required:

  • B.S. degree in Biochemistry, Cell/Molecular Biology, Bioprocess engineering or related fields and three (3) years of industry or academia experience or a M.S. degree with 1 year of experience in similar fields

Must haves:

  • Prior experience in mammalian cell culture techniques, and cell culture media preparation is a prerequisite: single use mammalian cell culture tools employed in bioprocess development, including but not limited to deep well plates and shake flasks.
  • Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).
  • Ability to multi-task different protocols at once, handle concurrent projects simultaneously with high attention to detail.

Nice to haves/preferred:

  • Experience with high-throughput systems and automation.
  • Experience with batch purification using a variety of scaffolds and basic QC.
  • Industry experience is preferred.

Software skills:

  • Basic knowledge on biological data collection and analysis tools (Vicell, metabolite analyzers) and computer applications such as ELN, MS Word, MS Excel and MS PowerPoint.
  • Experience with data and reagent management software such as Dotmatics, Benchling, Spotfire, etc is preferred.

Skills/techniques needed:

  • Excellent aseptic techniques and familiarity with principles of sterilization/disinfection.
  • Strong communication, organizational, and interpersonal skills.
  • Able to maintain a “can-do” attitude and act with a sense of urgency to complete innovative projects while being flexible in work priorities based on business needs.
  • Understand and adhere to corporate standards regarding code of conduct, safety, appropriate handling of materials
  • Able to troubleshoot cell culture system and think critically about experiments.
  • Must be comfortable with physical activities of constant standing, walking, and sitting with arm stretching into biosafety cabinets for cell culture work.

Responsibilities:

Department summary:

  • Our teams combine leading drug discovery capabilities and world-class R&D with the purpose of turning breakthrough science into life-changing medicines.
  • We are looking for a motivated scientist to be an integral part of the Protein Science department within Discovery Biologics at Client’s Research Labs- South San Francisco.
  • The successful candidate will be involved in multiple aspects of the production of recombinant proteins, monoclonal antibodies, multispecific scaffolds, and novel protein modalities to enable all aspects of Early Discovery.
  • Ideally, the candidate will be able to execute and develop on techniques involving recombinant protein expression in CHO cells using both transient and stable-pool techniques.
  • Additionally, the candidate should have foundational experience in purification and QC, along with a proven ability to accurately record and present data.

Job responsibilities in detail:

  • Maintain and propagate CHO cells in suspension culture independently to support ongoing projects in a cell culture lab.
  • Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293). Express and batch purify a variety of proteins (monoclonal, bispecific antibodies, VHHs, Fabs, Fc-Fusions, etc.) using both resin and magnetic-bead immunoprecipitation methods.
  • Execute fed-batch experiments in a variety of platforms: shake flasks 125ml to 5L, tube spins and multi-well plates.
  • Perform analytical measurements for cell growth, metabolite measurements and HPLC analysis of titers from supernatant.
  • Analyze purified proteins using techniques: SDS-PAGE, HPLC, UPLC, CESDS, Nano-drop, UV-Vis.
  • Maintain and troubleshoot mammalian cell culture systems.
  • Document protein production processes, prepare reports and communicate results
  • ELN documentation.

Key skills:

  • Maintain and propagate CHO cells in suspension culture independently
  • Perform transient and stable transfections for protein expression in mammalian cells(CHO cells).
  • Purification batch purification using bench top.
  • Candidate should have foundational experience in purification and QC

Job Tags

Flexible hours,

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